Preliminary isolation and in vitro antiyeast activity of active fraction from crude extract of Gracilaria changii

OBJECTIVE To isolate the active fraction from crude extract of Gracilaria changii and to determine its in vitro antifungal activity. MATERIALS AND METHODS The active fraction was isolated from the crude extract of G. changii by various purification procedures such as column chromatography, thin layer chromatography, bioauthograph etc. The in vitro antifungal activity (Candida albicans) of the active fraction (1.00, 0.50, and 0.25 mg/ml) was studied by disc diffusion method and the effect of the active fraction on the morphology of yeast was done by scanning electron microscope (SEM) studies. RESULTS An active fraction with remarkable antifungal activity was separated from the crude extract. The active fraction was effective as a fungicide against C. albicans and showed a dose-dependent antifungal activity. A Scanning Electron Microscope (SEM) study confirmed the fungicidal effect of G. changii active fraction on C. albicans, by changing the normal morphology of C. albicans. CONCLUSION From G. changii crude extract, an active fraction with remarkable in vitro antifungal activity has been isolated.